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Krishna Murari Sinha, Ph.D.
Institute of Molecular Medicine 254 Okhla Industrial Estate, Phase III New Delhi-110020, India Phone No: 011-41028710 Fax No: 011-41028709 Email: krishna@immindia.org Education: Ph.D. Indian Institute of Chemical Biology (Jadavpur University), India (1999) Postdoctoral Fellow, University of California, Los Angeles, USA (2000-2005) Career History: Mycobacterium tuberculosis is one of the most successful pathogens worldwide. About one third of the world population is infected with it and it causes about 3 million deaths every year. The unique biology of the bacteria is that they remain in latent stage within human body for long periods before reactivating and causing overt disease. The bacteria have to overcome numerous stresses within the body. The stress includes exposure to reactive oxygen and nitrogen species, low pH within macrophages, hypoxia etc. Bacteria have to overcome these different kinds of stresses for successful infection. They do so by inducing a cascade of very well organized stress responses. These stress responses have also been implicated in the evolution of drug resistant strains. Better understanding of these pathways will lead to insights into the strategies bacteria adopt to evade immune system and successful establishment of infection. My lab is interested in understanding the regulation of extracellular function sigma factors particularly the ones which are involved in regulating bacterial DNA replication and repair under stress. Sigma factors help bacteria in adapting to different environments. We will also be studying the bacterial proteins that are involved in DNA replication and repair and are induced during macrophage infection. Selected publications Sinha, K. M#., M. Unciuleac#, M. S. Glickman and S. Shuman 2009. AdnAB: a new DSB-resecting motor–nuclease from mycobacteria. Genes Dev (In Press) Sinha, K. M., M. S. Glickman and S. Shuman 2009. Mutational analysis of Mycobacterium UvrD1 identifies functional groups required for ATP hydrolysis, DNA unwinding, and chemomechanical coupling. Biochemistry 48, 4019-4030. Sinha, K. M., N. C. Stephanou, M. Unciuleac, M. S. Glickman. and S. Shuman. 2008. Domain requirements for DNA unwinding by Mycobacterial UvrD2, an essential DNA helicase. Biochemistry 47, 9355-9364. Sinha, K. M#., N. C. Stephanou#, F. Gao, Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair. J Biol Chem 282, 15114-15125. Sinha, K. M., J. C. Hines and D. S. Ray. 2006. Cell cycle dependent localization and properties of a second mitochondrial DNA ligase in Crithidia fasciculata. Eukaryotic Cell 5, 54-61. Downey, N., J. C. Hines, K. M. Sinha and D. S. Ray. 2005. Mitochondrial DNA ligases of Trypanosoma brucei. Eukaryotic Cell 4, 765-774. Sinha, K. M., J. C. Hines, N. Downey and D. S. Ray. 2004. Mitochondrial DNA ligases from Crithidia fasciculata and Trypanosoma brucei. Proc Natl Acad Sci USA. 101, 4361-4366. (Comment in Proc. Natl. Acad. Sci. USA. 101, 4333-4334) Mittra, B., K. M. Sinha, J. C. Hines and D. S. Ray. 2003. Presence of multiple mRNA cycling sequence element binding proteins in Crithidia fasciculata. J Biol Chem. 278, 26564-26571. Dutta, M#., P. Delhi#, K. M. Sinha, R. Banerjee and A. K. Datta. 2001. Lack of abundance of cytosolic cyclosporin A-binding protein renders free-living Leishmania donovani resistant to cyclosporin A. J Biol Chem 276, 19294-19300. Sinha, K. M., M. Ghosh, I. Das and A. K. Datta. 1999. Molecular cloning and expression of adenosine kinase from Leishmania donovani: identification of unconventional P-loop motif. Biochem J 339, 667-673. | 
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