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R.K. Sunil Singh, Ph.D
Institute of Molecular Medicine254 Okhla Industrial Estate, Phase III New Delhi: 110020, India Phone: 011-41028710 Fax: 011-41028709 254, Okhla Industrial Estate, Phase 3 Email: rajkumars@tcgaresearch.org Education: Ph.D Bio-organic Chemistry, Indian Institute of Chemical Technology (IICT), Hyderabad, India (1999-2004). M.Sc. Organic Chemistry, Jadavpur University, Kolkata, India (1996-1998). B.Sc. Chemistry (Hons), Jadavpur University, Kolkata, India (1993-1996). Career History: Oct 2008-present: Research Scientist, Institute of Molecular Medicine, New Delhi, India. 2004-2008: Post-Doctoral Fellow, Pharmacology, University of California, Los Angeles, USA. Research activities: Studying apoptosis using a chemical biology approach: Our lab is interested in discovering novel cancer cells selective apoptotic agents and understanding apoptosis signaling pathways using a chemical biology approach. A common characteristic of most cancer cells is the ability to evade apoptosis, the natural death process or programmed cell death. Normal healthy cells have a tightly regulated mechanism for apoptosis but cancer cells often have multiple means by which they evade this pathway and undergo uncontrolled cell proliferation. Agents capable of restoring the normal apoptotic pathways in cancer cells have the potential for effectively treating many cancers. In the first approach, we will generate a diverse set of small molecule libraries using combinatorial chemistry techniques, screen the compound collections in mammalian cells for apoptosis (or non-apoptotic) induction and use these apoptosis inducers for further mechanistic study on regulation of cell death. Using probe reagents prepared from the initial lead apoptosis inducers, cellular target identification of the bioactive molecules and signaling pathways affected by the compounds will be carried out through a combination of biochemical, genomics and organic chemistry techniques. The overall aim of this approach is to identify tumour cells selective apoptotic agents and also to gain mechanistic insights into the differential regulation of cell death (apoptosis in particular) in normal and cancer cells. In the second approach, we will design and identify apoptosis inducing small interfering RNAs by targeting several cancer gene targets. Examples of potential apoptotic targets include the epidermal growth factor receptors, anti-apoptotic Bcl-2 and inhibitors of proteins (IAPs) which are overexpressed in many types of cancer. siRNAs capable of inducing apoptosis in appropriate cell culture models will be identified, validated and chemically modified to increase their potency and nuclease stability. This study will help identify and develop anti-cancer siRNAs for potential therapeutic applications. (Funding: DST) Publications:Rajkumar, Sunil. Singh., J. Dixon. Wendy and Han Htun 2008. Distinct patterns of factor accumulation at a hormone-responsive locus provide visual evidence for chromatin looping in living mammalian cells (under review). Rajkumar, Sunil. Singh., Goncalves. Christine, Sabdrin. Pierre, Pichon. Chantal, Midoux Patrick. and Arabinda. Chaudhuri 2004. On the Gene Delivery efficacies of pH-sensitive Cationic lipids via endosomal Protonation: A Chemical Biology Investigation Chemistry & Biology 11: 713-23. Bharat, Kumar. Majeti., Rajkumar. Sunil. Singh., Sudheer. Kumar, Surender. Yadav, Bathula. Reddy, Ramakrishna. Sistla, Vamanrao. Prakash, Surkara. Diwan, Madhvendra. Sakunthala and Arabinda Chaudhuri 2004. Enhanced Intravenous Transgene expression in Mouse Lung using Cyclic-head Cationic Lipids Chemistry & Biology 11:427-37 (equal contributing authors). Rajkumar, Sunil. Singh and Arabinda Chaudhuri 2004. Single additional methylene group in the head-group region imparts high gene transfer efficacy to a transfection-incompetent cationic lipid FEBS Letters 556: 86-90. Valluripalli, Vinod. Kumar., Rajkumar. Sunil. Singh and Arabinda. Chaudhuri 2003. Cationic Transfection Lipids in Gene Therapy: Successes, Setbacks, Challenges and Promises Current Medicinal Chemistry 10: 1297-1306. Rajkumar, Sunil. Singh., Koushik. Mukherjee, Rajkumar. Banerjee, Arabinda. Chaudhuri, Samik. Kumar, Hait. Satya, Priya. Moulik, Yerramsetti. Ramadas, Amash. Vijayalakshmi and Nalam. Madhusudhana. Rao 2002. Anchor Dependency for Non-glycerol Based Cationic Lipofectins: Mixed bag of Regular and Anomalous Transfection Profiles Chemistry-A European Journal 8: 900-908. Saumitra, Sengupta., Subir. Kumar. Sadhukhan, Rajkumar. Sunil. Singh and Nilashish. Pal 2002. Synthesis of Dendritic Stilbenoid Compounds: Heck reaction for Periphery and the Core Tetrahedron Letters 43: 1117-1121. Saumitra, Sengupta, Subir. Kumar. Sadhukhan and Rajkumar. Sunil. Singh 2002. A Palladium Catalyzed Synthesis of Wheel and Axle Hydrocarbons: Strategic Use of Tetraphenylmethane Stoppers 2002. Indian J Chem Sec B, 41B: 642-644. Saumitra, Sengupta., Subir. Kumar. Sadhukhan and Rajkumar. Sunil. Singh 2001. Heck reactions of arenediazonium salts with phenyl vinyl sulfones: A new synthesis of styryl sulfones Indian J Chem SecB 40B: 997-999. | 
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